- blotting hybridization
- блот-гибридизация, блоттинг-гибридизация, блот-анализ
English-Russian Biotechnology Glossary. A. F. Valikhov, V. V. Kolotvin, O. A. Legonkova, I. A. Rogov, Thomas Creamer. 2007.
blotting hybridization
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Hybridization probe — In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length (usually 100 1000 bases long), which is used in DNA or RNA samples to detect the presence of nucleotide sequences (the DNA target) that are complementary… … Wikipedia
blotting — General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel to a paper like membrane (usually nylon or nitrocellulose) by capillarity or an electric field, preserving the spatial arrangment.… … Dictionary of molecular biology
Nucleic acid hybridization — Hybridization is the process, discovered by Alexander Rich, of combining complementary, single stranded nucleic acids into a single molecule. Nucleotides will bind to their complement under normal conditions, so two perfectly complementary… … Wikipedia
filter hybridization — any of various methods, e.g., Southern blotting, dot blotting, for detection of a specific nucleic acid sequence by immobilizing a population of single stranded nucleic acids on a filter, exposing it to a specific labeled single stranded nucleic… … Medical dictionary
Southern blotting technique — The procedure used to isolate and identify DNA fragments from a complex mixture. The isolated, denatured fragments are transferred from an agarose electrophoretic gel to a nitrocellulose filter and identified by hybridization with probes … Dictionary of microbiology
Restriction fragment length polymorphism — A Restriction fragment length polymorphism (or RFLP, often pronounced as rif lip ) is a variation in the DNA sequence of a genome that can be detected by breaking the DNA into pieces with restriction enzymes and analyzing the size of the… … Wikipedia
Northern blot — The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.[1][2] … Wikipedia
Molecular biology — (pronounced /məˈlɛkjʊlər .../) is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology… … Wikipedia
Southern blot — A Southern blot is a method routinely used in molecular biology to check for the presence of a DNA sequence in a DNA sample. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size… … Wikipedia
Medical genetics — Clinical genetics redirects here. For the journal, see Clinical Genetics (journal). For a non technical introduction to the topic, see Introduction to Genetics. Part of a series on Genetics Key components Chromosome … Wikipedia
Gene expression — For vocabulary, see Glossary of gene expression terms. For a non technical introduction to the topic, see Introduction to genetics. Genes are expressed by being transcribed into RNA, and this transcript may then be translated into protein. Gene… … Wikipedia
